Fig 2: Cortical dendritic differentiation is affected in mice carrying decreasing amounts of functional Cebpa/b alleles. (a-f) Cresyl violet staining showing a normal lamination pattern in newborn mutant and control mice. (g,i,k) Representative western blots of NeuN, Tju1, and GFAP protein levels in P0 cortices of mutant (Cebpalx/lx;Cebpb+/-;NesCretg/+) and control (Cebpalx/lx;Cebpb+/-) mice. To control for protein loading, blots were re-probed with anti-PLCγ, anti-α-tubulin, and anti-Erk1 respectively. (h,j,l) Densitometric analysis of NeuN, Tju1, and GFAP protein levels in P0 cortical lysates from mutant and control mice. P > 0.05 in all cases. (m-r) MAP2 immunostaining in newborn Cebpalx/lx, Cebpalx/lx;NesCretg/+, Cebpb-/-, and Cebpalx/lx;cebp+/- mouse cortices compared to cortices with decreasing amounts of functional Cebpa/b alleles, Cebpalx/lx;Cebpb+/-;NesCretg/+ (r), Cebpalx/+;Cebpb-/-;NesCretg/+ (s). (s,u) Representative western blots of MAP2 and NF68 protein levels in P0 cortices of mutant (Cebpalx/lx;Cebpb+/-;NesCretg/+) and control (Cebpalx/lx;Cebpb+/-) mice. To control for protein loading, blots were re-probed with anti-PLCγ and anti-Erk1 antibodies respectively. (t) Densitometric analysis of MAP2 protein levels in cortical lysates of control and Cebpa/b mutant mice (*p = 0.002, compared to control Cebpalx/lx;Cebp+/-). (v) Densitometric analyses of NF68 protein levels in cortical lysates of controls and Cebpa/b mutant mice. Soms, somatosensory cortex; CP, cortical plate; V and VI, indicate cortical layers V and VI, respectively. Arrows in (h) indicate the kDa of the bands recognized by NeuN antibodies. Scale bars: 100 μm (b-g); 50 μm (n-s).